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In Vitro Study No: 359

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
4-Iodophenol 0.005-0.010.004EthanolRatDermatomed to 0.35mmDorsal0.32Flow-throughEagles MEM. 10% BSA Not Given72721Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
100.1  15.321.7 95 73.316   10 

NOTES

Cells were covered with parafilm to limit evaporation. 9-12 cells were used. The % penetrated was calculated by adding the % found in the receptor fluid to the % found in the skin.

REFERENCE

Comparative In Vitro Percutaneous Absorption Of P-Substitued Phenols Through Rat Skin Usimg Static And Flow-through Diffusion Systems (1993) Toxicol. Vitro, 7 :221-227