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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 368

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Propoxur

0.05

aq. Methanol

Human

Full Thickness Skin

Abdomen

0.64

Static

RPMI, L-glutamine, gentamycin & 10% foetal calf serum

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
89	4.1		5.8	31.6		33.6		2	0.3				5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

4.1

5.8

31.6

33.6

0.3

NOTES

6-well tissue culture plates were used in a humidified incubator.This result is achieved from a combination of experiments where different concentrations of Propoxur were added. 0.025, 0.1 and 0.2 mg/cm2

REFERENCE

Species Specific Cutaneous Biotransformation Of The Pesticide Propoxur During Percutaneous Absorption In Vitro (1993) Toxicol. Appl. Pharmacol., 123 :144-150