IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 371
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Propoxur
0.32
0.05
0.025
aq. Methanol
Rabbit
Full Thickness Skin
Dorsal
0.64
Static
RPMI, L-glutamine, gentamycin & 10% foetal calf serum
37
Incubator
24
26
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.0108
0.0044
NOTES
6-well tissue culture plates were used in a humidified incubator
REFERENCE
Species Specific Cutaneous Biotransformation Of The Pesticide Propoxur During Percutaneous Absorption In Vitro (1993) Toxicol. Appl. Pharmacol., 123 :144-150