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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 377

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Captopril10Infinite Water & 5% Benzalkonium ChlorideRabbitFull Thickness SkinAbdomen2StaticpH 7.4 McIlvaine buffer37Receptor Fluid48481HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          157.64147.26 35.61

NOTES

Cells were occluded with parafilm. Most of the experimental detail has been obained from an earlier paper. Int J Pharm 126 (1995) 119-128. Results were given as +/- SE. I have converted these to SD for this database. 6921.33 microg/cm2 was found in the receptor fluid after 48h

REFERENCE

In Vitro Percutaneous Absorption Of Captopril Through Excised Rabbit Skin (1996) Int. J. Pharm., 143 :119-123