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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 382

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Captopril

Infinite

Water & 5% Tween 60

Rabbit

Full Thickness Skin

Abdomen

Static

pH 7.4 McIlvaine buffer

Receptor Fluid

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										27.56	14.79		3	6.47

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

27.56

14.79

6.47

NOTES

Cells were occluded with parafilm. Most of the experimental detail has been obained from an earlier paper. Int J Pharm 126 (1995) 119-128. Results were given as +/- SE. I have converted these to SD for this database. 1384.40 microg/cm2 was found in the receptor fluid after 48h

REFERENCE

In Vitro Percutaneous Absorption Of Captopril Through Excised Rabbit Skin (1996) Int. J. Pharm., 143 :119-123