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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 384

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzene1.753Infinite WaterHumanIsolated EpidermisAbdomen Static0.1% NaCl solution31Skin441HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.19283 0.11113 

NOTES

Cadaver Skin was used. Epidermal sheets were prepared by immersing the skin in water at 60oC for 30 s. Cells were tightly capped at all times to prevent evaporation. Saturated donor solutions of benzene were used. Concentration was given as 2microlitres/ml. Density of Benzene is 876.5mg/ml

REFERENCE

Penetration Of Benzene Through Human Skin (1985) J. Investig. Dermatol., 85 :522-526