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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 385

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzene

43.825

Infinite

Hexodecane

Human

Isolated Epidermis

Abdomen

Static

0.1% NaCl solution

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.0438		0.00094	14

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0438

0.00094

NOTES

Cadaver Skin was used. Epidermal sheets were prepared by immersing the skin in water at 60oC for 30 s. Cells were tightly capped at all times to prevent evaporation. Saturated donor solutions of benzene were used. Concentration was given as 2microlitres/ml. Density of Benzene is 876.5mg/ml

REFERENCE

Penetration Of Benzene Through Human Skin (1985) J. Investig. Dermatol., 85 :522-526