IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 389
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Water
1000
0.15
375
None
Human
Dermatomed to 0.42mm
Abdomen
0.4
Flow-through
0.002% Sodium Azide Solution
31
Skin
6
6
1
Radiolabelled (3H)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.0013
9
NOTES
Donor Compartments were covered. Results are for the first day of hydration of the skin
REFERENCE
Shed Snake Skin And Hairless Mouse Skin As Model Membranes For Human Skin During Permeation Studies (1990) J. Investig. Dermatol., 94 :235-240
