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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 395

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Water

1000

0.15

375

None

Mouse

Full Thickness Skin

Dorsal

0.4

Flow-through

0.002% Sodium Azide Solution

Skin

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00219	10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00219

NOTES

Donor Compartments were covered. Hairless mouse skin was used. Results are for the first day of hydration of the skin

REFERENCE

Shed Snake Skin And Hairless Mouse Skin As Model Membranes For Human Skin During Permeation Studies (1990) J. Investig. Dermatol., 94 :235-240