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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 4

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

25.82

Water

Human

Isolated Epidermis

Upper Leg

Static

PBS

Receptor Fluid

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										6.65	0.16	0.000258	4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

6.65

0.16

0.000258

NOTES

This was a saturated solution of Caffeine. They also tested other commercially available fornulations. The steady state flux was measured between 8 and 24 hours after steady state had been achieved.. The Kp was derived from this.

REFERENCE

Topical Delivery Of Caffeine From Some Commercial Formulations (1999) Int. J. Pharm., 182 :41-47