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In Vitro Study No: 40

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Salicylic acid0.010.30.001290% aq. Propylene glycolSkinethic TM filter support was left attachedN/A2.54StaticPBS/Ethanol 3:132Water Bath48482reversed phase HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          152.815.715.283 

NOTES

Obtained from Skinethic (Nice, France). No integrity test was mentioned. They also measured the concentration of chemical remaining in the skin after the stratum cormeum had been stripped. They found this to be 745+/-216 micrograms/gram. The Kp was not mentioned in the paper. I calculated it by dividing the steady state flux by the concentration

REFERENCE

Comparison Of Human Skin Or Epidermis Models With Human And Animal Skin In In Vitro Percutaneous Absorption (2001) Int. J. Pharm., 215 :51-56