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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 463

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Ethanol

1.4

0.9% Saline

Mouse

Full Thickness Skin

Abdomen/Dorsal

0.6

Static

Saline

Not Given

20.5

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.0021	8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0021

NOTES

Hairless Mice were used. The half cell was filled with 1.4ml Saline then 0.2ml was removed and then 0.1ml of a stock solution of radioactive Ethanol (1E-04 M or less) and 0.1ml of radioactive Water were added Skin was used for more than one run, though they were checked to make sure that there was no residual activity between runs

REFERENCE

Hydration And Percutaneous Absorption: I. Influence Of Hydration On Alkanol Permeation Through Hariless Mouse Skin (1980) J. Investig. Dermatol., 75 :346-352