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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 5

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

See Notes

0.09

Loreal anti-cellulite cream

Human

Dermatomed to 0.344mm

Abdomen/Breast

1.86

Static

aq. BSA, NaCl, KCl & gentamycin sulphate

Receptor Fluid

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
94.7	3	2.56		0.22	2.22	4		1.22	0.89				9

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

94.7

2.56

0.22

2.22

1.22

0.89

NOTES

The skin was from Caucasian Females. The Caffeine was applied in the form of an anti-cellulite cream containing 3% Caffeine. 3mg/cm2 of the cream was applied.

REFERENCE

Quantitative HPLC Analysis Of Sunscreens And Caffeine During In Vitro Percutaneous Penetration Studies (1999) Int. J. Pharm., 189 :249-260