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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 547

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-Phenoxyethanol

10.7

0.02

0.271

Methanol

Rat

Dermatomed to 0.244mm

Dorsal

0.79

Static

aq. Ethanol

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
68		0.87	0.61	1.03	0.48	65.5		63.6	11.6			2.72E-05	7

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.87

0.61

1.03

0.48

65.5

63.6

11.6

2.72E-05

NOTES

Male Wistar Rats 28-35 days old were used.1550nmol of 2-phenoxyethanol was added in total. Results were given as +/- SEM. I have changed this to SD for this database. % Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the epidermis, dermis and stratum corneum.

REFERENCE

A Comparison Of The Absorption Of A Series Of Ethoxylates Through Rat Skin In Vitro (1998) Toxicol. Vitro, 12 :57-65