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In Vitro Study No: 551

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Dodecyl decaethoxylate80.020.203MethanolRatDermatomed to 0.244mmDorsal0.79Staticaq. Ethanol37Receptor Fluid24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
95 4.730.784.210.8158.34 49.418.2  0.0004296 

NOTES

Male Wistar Rats 28-35 days old were used.332nmol of Dodecyl Decaethoxylate was added in total. Results were given as +/- SEM. I have changed this to SD for this database. % Penetrated was calculated by6 adding the amount found in the receptor fluid to the amount found in the epidermis, dermis and stratum corneum.

REFERENCE

A Comparison Of The Absorption Of A Series Of Ethoxylates Through Rat Skin In Vitro (1998) Toxicol. Vitro, 12 :57-65