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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 552

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dodecyl decaethoxylate

10.7

0.02

0.25

Methanol

Rat

Dermatomed to 0.244mm

Dorsal

0.64

Flow-through

MEM & Penicilin & streptomycin

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
81		5.45	5.9	8.92	1.18	21.38		7.01	4.28			4.89E-06	4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

5.45

5.9

8.92

1.18

21.38

7.01

4.28

4.89E-06

NOTES

Male Wistar Rats 28-35 days old were used.332nmol of Dodecyl Decaethoxylate was added in total. Results were given as +/- SEM. I have changed this to SD for this database.% Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the epidermis, dermis and stratum corneum.

REFERENCE

A Comparison Of The Absorption Of A Series Of Ethoxylates Through Rat Skin In Vitro (1998) Toxicol. Vitro, 12 :57-65