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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 554

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Lindane

120

10.5

1.6

Acetone

Human

Dermatomed to 0.23mm

Breast/Abdomen

0.79

Static

50% aq. Ethanol

Skin

Gas Chromatography

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
		27.5	0.29	0.4	27.9	28.59		0.69	0.147	0.71	0.29		6

NOTES

The skin was swabbed after 6h but measurements were made up until 24h. The % Absoprtion was given as +/- SEM. I have converted this to SD for this database. % Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the skin and stratum corneum.

REFERENCE

The Percutaneous Absorption And Skin Distribution Of Lindane In Man. II. In Vitro Studies (1997) Hum. Exp. Toxicol., 16 :652-657