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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 561

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzoic Acid

See Notes

0.0005

Petrolatum

Human

Dermatomed to 0.35mm

Abdomen

0.64

Flow-through

Saline

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								46.5	14.45				6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

46.5

14.45

NOTES

3.2mg of petrolatum was added in total. (5mg/cm2). The results were given +/- SEM. I have changed this to SD for this database.

REFERENCE

Vehicle Effects On Percutaneous Absorption: In Vivo And In Vitro Comparison With Human Skin (1986) Br. J. Dermatol., 115 :1-11