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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 563

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

See Notes

0.002

Petrolatum

Human

Dermatomed to 0.35mm

Abdomen

0.64

Flow-through

Saline

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								61.8	10.4			0.00072	12	1.2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

61.8

10.4

0.00072

1.2

NOTES

16mg of petrolatum was added in total. (25mg/cm2). The results were given +/- SEM. I have changed this to SD for this database. The skin came from at least 2 donors. Cadaver skin was used.

REFERENCE

Vehicle Effects On Percutaneous Absorption: In Vivo And In Vitro Comparison With Human Skin (1986) Br. J. Dermatol., 115 :1-11