IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 564
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Caffeine
See Notes
0.06
Petrolatum
Human
Dermatomed to 0.35mm
Abdomen
0.64
Flow-through
Saline
32
Skin
36
36
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
40.6
5.82
0.00062
7
1.1
NOTES
32mg of petrolatum was added in total. (50mg/cm2). The results were given +/- SEM. I have changed this to SD for this database. The skin came from at least 2 donors. Cadaver skin was used.
REFERENCE
Vehicle Effects On Percutaneous Absorption: In Vivo And In Vitro Comparison With Human Skin (1986) Br. J. Dermatol., 115 :1-11