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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 580

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Mannitol1Not Given Distilled WaterPigIsolated EpidermisOuter Ear2.54Static0.9% physiological saline Water Bath  2Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.00023443 

NOTES

Pig Ears were obtained from the abattoir and were cleaned and shaved before use..Get more experimental detail from Fundamental and Applied Toxicology (1992) 19 484-492

REFERENCE

Skin Absorption: Flow-Through Or Static Diffusion Cells (1994) Toxicol. Vitro, 8 :827-830