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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 581

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Mannitol1Not Given Distilled WaterPigIsolated EpidermisOuter Ear0.64Flow-through0.9% physiological saline32Water Bath  2Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.0015213 

NOTES

Pig Ears were obtained from the abattoir and were cleaned and shaved before use. Get more experimental detail from Fundamental and Applied Toxicology (1992) 19 484-492

REFERENCE

Skin Absorption: Flow-Through Or Static Diffusion Cells (1994) Toxicol. Vitro, 8 :827-830