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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 6

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid0.03050.050.00238EthanolRatIsolated EpidermisBack0.64Flow-through50% aq. Ethanol31.5Skin30302Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        68.317.4  0.0286111.33

NOTES

The flow rate in the cell was 1.9ml/min. The skin was taken from 4-5 wk old male Ola:Sprague Dawley rats it was soaked in 2M Sodium Bromide for 24 hours prior to the study to separate the epidermis and dermis.

REFERENCE

Absorption Of Hydrophilic And Lipophilic Compounds Through Epidermal And Subepidermal Strata Of Rat Skin in Vitro (1998) Toxicol. Vitro, 12 :47-55