IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 610
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Nicotinic Acid
Not Given
0.0024
Ethanol
Rat
Full Thickness Skin
Dorsal
0.32
Flow-through
HEPES buffered HBSS & 0.5% gentamycin
32
Skin
72
72
1
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
89.6
5.2
7.9
58.4
67
8.6
0.8
2
NOTES
Occluded with Teflon caps 2-3 parallel cells were run. % Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the skin.
REFERENCE
Percutaneous Absorption Of Nicotinic Acid, Phenol, Benzoic Acid And Triclopyr Butoxyethanol Ester Through Rat And Human Skin In Vitro: Further Validation Of An In Vitro Model By Comparison With In Vivo Data (1992) Food Chem.Toxicol., 30 :891-899