EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 610

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Nicotinic Acid Not Given0.0024EthanolRatFull Thickness SkinDorsal0.32Flow-throughHEPES buffered HBSS & 0.5% gentamycin32Skin72721Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
89.65.2 7.958.4 67 8.60.8   2 

NOTES

Occluded with Teflon caps 2-3 parallel cells were run. % Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the skin.

REFERENCE

Percutaneous Absorption Of Nicotinic Acid, Phenol, Benzoic Acid And Triclopyr Butoxyethanol Ester Through Rat And Human Skin In Vitro: Further Validation Of An In Vitro Model By Comparison With In Vivo Data (1992) Food Chem.Toxicol., 30 :891-899