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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 612

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Nicotinic Acid

Not Given

0.0021

Ethanol

Rat

Full Thickness Skin

Dorsal

0.32

Flow-through

HEPES buffered HBSS & 0.5% gentamycin

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
100.2	2.2		9.6	66.5		72.5		6	1.2				2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

100.2

2.2

9.6

66.5

72.5

1.2

NOTES

2-3 parallel cells were run.% Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the skin.

REFERENCE

Percutaneous Absorption Of Nicotinic Acid, Phenol, Benzoic Acid And Triclopyr Butoxyethanol Ester Through Rat And Human Skin In Vitro: Further Validation Of An In Vitro Model By Comparison With In Vivo Data (1992) Food Chem.Toxicol., 30 :891-899