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In Vitro Study No: 619

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Phenol Not Given0.0027EthanolHumanFull Thickness SkinBreast0.32Flow-throughHEPES buffered HBSS & 0.5% gentamycin32Skin72721Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
81.89.5 0.77.3 54.4 47.10.5   3 

NOTES

Healthy human breast Skin was obtained at surgery. Cells were occluded with Teflon Caps. % Penetrated was calculated by adding the amount found in the receptor fluid to the amount found in the skin.

REFERENCE

Percutaneous Absorption Of Nicotinic Acid, Phenol, Benzoic Acid And Triclopyr Butoxyethanol Ester Through Rat And Human Skin In Vitro: Further Validation Of An In Vitro Model By Comparison With In Vivo Data (1992) Food Chem.Toxicol., 30 :891-899