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In Vitro Study No: 635

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Theophylline0.650.050.00325EthanolPigFull Thickness SkinNot Given10NoneWhole Pigs Blood Not Given882Radilabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
804.1233.611.2734.8 0.60.8   5 

NOTES

this was an isolated perfused porcine skin flap model. For More Experimental Detail get Am J Vet Res 1991 Apr;52(4):75-82. The % Penetrated was calculated by adding the amounts found in the perfusate to the amounts found in the skin and stratum corneum

REFERENCE

Percutaneous Absorption Of Salicylic Acid, Theophylline, 2,4-Dimethylamine, Diethyl Hexyl Phthalic Acid, And p-Aminobenzoic Acid In The Isolated Perfused Porcine Skin Flap Compared To Man In Vivo (1998) Toxicol. Appl. Pharmacol., 151 :159-165