IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 637
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
DEHP
3.7
0.05
0.0185
Ethanol
Pig
Full Thickness Skin
Not Given
10
None
Whole Pigs Blood
Not Given
8
8
2
Radilabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
94.1
4.7
14.5
2.5
3.7
5.5
18.34
0.14
0.1
5
NOTES
This was an isolated perfused porcine skin flap model. For More Experimental Detail get Am J Vet Res 1991 Apr;52(4):75-82. % Penetrated was calculated by adding the amounts found in the perfusate to the amounts found in the skin and stratum corneum
REFERENCE
Percutaneous Absorption Of Salicylic Acid, Theophylline, 2,4-Dimethylamine, Diethyl Hexyl Phthalic Acid, And p-Aminobenzoic Acid In The Isolated Perfused Porcine Skin Flap Compared To Man In Vivo (1998) Toxicol. Appl. Pharmacol., 151 :159-165