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In Vitro Study No: 638

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2,4 Dimethylamine7.940.050.0397EthanolPigFull Thickness SkinNot Given10NoneWhole Pigs Blood Not Given882Radilabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
31.25.64.80.482.32.68.6 1.50.24   5 

NOTES

This was an isolated perfused porcine skin flap model. For More Experimental Detail get Am J Vet Res 1991 Apr;52(4):75-82. % Penetrated was calculated by adding the amounts found in the perfusate to the amounts found in the skin and stratum corneum. 2 different dosing amounts were given in error in the paper, I have taken this to be the correct one, but another value of 8.7 micrograms per cm2 was given in the materials and methods

REFERENCE

Percutaneous Absorption Of Salicylic Acid, Theophylline, 2,4-Dimethylamine, Diethyl Hexyl Phthalic Acid, And p-Aminobenzoic Acid In The Isolated Perfused Porcine Skin Flap Compared To Man In Vivo (1998) Toxicol. Appl. Pharmacol., 151 :159-165