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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 641

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Fluazifop-butyl

0.5

0.0125

0.0025

Commercial Emulsifyable Concentrate

Human

Isolated Epidermis

Abdomen

2.5

Static

6% propylene glycol 20 oleyl ether in saline

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0.32	0.08

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.32

0.08

NOTES

Membrane integrity was assessed by determining the permeability of tritiated water.

REFERENCE

The Predictive Accuracy Of In Vitro Measurements For The Dermal Absorption Of A Lipophilic Penetrant (Fluazifop-Butyl) Through Rat And Human Skin (1994) Fundam. Appl. Toxicol., 23 :230-236