IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 644
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Fluazifop-butyl
5
0.0125
0.025
Commercial Emulsifyable Concentrate
Human
Isolated Epidermis
Abdomen
2.5
Static
propylene glycol 20 oleyl ether in saline
30
24
24
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.472
0.272
NOTES
Membrane integrity was assessed by determining the permeability of tritiated water.
REFERENCE
The Predictive Accuracy Of In Vitro Measurements For The Dermal Absorption Of A Lipophilic Penetrant (Fluazifop-Butyl) Through Rat And Human Skin (1994) Fundam. Appl. Toxicol., 23 :230-236