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In Vitro Study No: 666

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Butachlor3.740.00460.0174EthanolHumanDermatomed to 0.5mmNot Given1Flow-throughMEM & gentamycin sulphate & 10% Fetal Bovine Serum37Receptor Fluid24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
96.39.15.30.23.21.29.3 0.80.7   3 

NOTES

The skin samples cam efrom the cadaver of a 33 year old caucasian male. The skin was still viable. % Penetrated was calculated by adding together the amount found in the receptor fluid and the amount that had entered the skin, including the stratum corneum. In the paper they did this slightly differently, they included the amount that was found solubilised in the skin as absorbed (3.2%) but did not include the amount that remained in the solid precipitate after the skin was homogenized (5.3%) Concentration was given as 12 picomoles/microlitre. I converted this to mg/ml for the database.

REFERENCE

Absorption And Metabolism Of 2-Chloro-2,6-Diethyl-N-(butoxymethyl)acetanilide (Butachlor) In Human Skin In Vitro (1993) Toxicol. Appl. Pharmacol., 121 :78-86