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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 67

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Testosterone

0.0048

0.0096

Water

Testskin TM

3microm pore size polycarbonate membrane

N/A

0.5

Static

LDE Assay Medium & gentamycin sulphate

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.1392		0.029	12

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.1392

0.029

NOTES

0.5 microCi and 2microg of testosterone in each ml of water. The Kp was calculated from the average % applied dose/cm2/h for the 2nd through to the 6th h, when rates had reached steady state. They didn't include the steady state flux in the paper. I calculated it by multiplying the Kp by the concentration.They used 3 LSE's per exp 3-6 exp.

REFERENCE

Absorption And Metabolism Of Topically Applied Testosterone In An Organotypic Skin Culture (1992) Skin Pharmacol., 5 :146-153