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In Vitro Study No: 671

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Coumarin0.20.0050.0037EthanolRatFull Thickness SkinDorsal0.32Flow-throughHEPES buffered HBSS & 0.5% gentamycin32Skin72722Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
77.77.6 512.7 64 51.37.30.157  120.2

NOTES

Male Fischer 344 Rats were used. 3 replicates of skin from 4 Rats was used in this study.

REFERENCE

Comparative In Vitro Skin Absorption And Metabolism Of Coumarin (1,2-Benzopyrone) In Human, Rat and Mouse (1997) Toxicol. Appl. Pharmacol., 145 :34-42