IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 672
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Coumarin
0.2
0.005
0.0037
Ethanol
Mouse
Full Thickness Skin
Dorsal
0.32
Flow-through
HEPES buffered HBSS & 0.5% gentamycin
32
Skin
72
72
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
83.9
13.6
31.7
76.6
44.9
13.5
0.1996
18
0.2
NOTES
3 replicates of skin from 6 micewas used in this study.
REFERENCE
Comparative In Vitro Skin Absorption And Metabolism Of Coumarin (1,2-Benzopyrone) In Human, Rat and Mouse (1997) Toxicol. Appl. Pharmacol., 145 :34-42