EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 677

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Propoxur

1.92

0.05

0.15

60% aq. Ethanol

Human

Full Thickness Skin

Abdomen

0.64

Static

Culture Medium & 10% Fetal Bovine Serum

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								9.67	2.27	1.7		0.000885	6	2.3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

9.67

2.27

1.7

0.000885

2.3

NOTES

The integrity of the skin was tested with [3H]labelled water. The Potentially absorbed dose was calculated from the applied dose minus the dose left on the surface at the end of the experiment this was 16.9 mcrg/cm2. The % Absorption after 4 hours was 1.73+/-0.47. The permeability coefficient was calculated by dividing the steady state flux by the concentration applied.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Absorption Of The Pesticide Propoxur (2000) Toxicol. Sci., 58 :15-22