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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 678

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Propoxur

1.92

0.05

0.15

60% aq. Ethanol

Human

Isolated Epidermis

Abdomen

0.64

Static

Saline (0.9%NaCl w/v) & 0.01% sodium azide & BSA

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
						39.2		25.87	6.93	4.9		0.0025521	6	1.1

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

39.2

25.87

6.93

4.9

0.0025521

1.1

NOTES

The epidermal membrane was separated by immersion of the skin in water heated to 60oC. The integrity of the skin was tested with [3H]labelled water. The Potential absorbed dose was calculated from the applied dose minus the dose left on the surface at the end of the experiment, this was 58.8mcrg/cm2. The % Absorption after 4 hours was 9.8+/-3.93. The permeability coefficient was calculated by dividing the steady state flux by the concentration applied.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Absorption Of The Pesticide Propoxur (2000) Toxicol. Sci., 58 :15-22