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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 681

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Propoxur

7.5

0.2

0.15

60% aq. Ethanol

Pig

Full Thickness Skin

Ear

Perfused Pig Ear

Whole Pigs Blood

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								5.33	3.2	2.5		0.000333	6	1

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

5.33

3.2

2.5

0.000333

NOTES

The integrity of the skin was tested with [3H]labelled water. The Potential absorbed dose was calculated from the applied dose minus the dose left on the surface at the end of the experiment this was 26.1 mcrg/cm2. The absorbed dose after 4h was 8mcrg/cm2. The permeability coefficient was calculated by dividing the steady state flux by the concentration applied.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Absorption Of The Pesticide Propoxur (2000) Toxicol. Sci., 58 :15-22