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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 683

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-Nitro-1,4-Benzenediamine

0.313

0.0096

0.0047

Ethanol

Human

Not Given

Abdomen

0.64

Flow-through

HHBSS pH 7.4

Not Given

0.5

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
72.6	1.27		3.17	3.9		9.2	5.75	5.3	2.57				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

72.6

1.27

3.17

3.9

9.2

5.75

5.3

2.57

NOTES

Barrier integrity of the skin was checked with tritiated water. For further Experimental Detail see J. Soc. Cosmet. Chem. 48 187-197 (1997)

REFERENCE

Percutaneous Penetration And Metabolism Of 2-Nitro-p-phenylenediamine In Human And Fuzzy Rat Skin (2000) Toxicol. Appl. Pharmacol., 166 :13-23