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In Vitro Study No: 684

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2-Nitro-1,4-Benzenediamine See Notes0.0047Semi-Permenant Hair Dye FormulationHumanNot GivenAbdomen0.64Flow-throughHHBSS pH 7.4 Not Given0.5242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
63.80.31 1.62.5 9.53.167.11.56   8 

NOTES

The semi-permenant Hair dye formulation was applied at a dose of 7.8mg/cm2. Barrier integrity of the skin was checked with tritiated water. For further Experimental Detail see J. Soc. Cosmet. Chem. 48 187-197 (1997)

REFERENCE

Percutaneous Penetration And Metabolism Of 2-Nitro-p-phenylenediamine In Human And Fuzzy Rat Skin (2000) Toxicol. Appl. Pharmacol., 166 :13-23