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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 688

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-Nitro-1,4-Benzenediamine

See Notes

0.0047

Control Diluted Permenant hair dye (no H2O2)

Rat

Not Given

0.64

Flow-through

HHBSS pH 7.4

Not Given

0.5

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
71.6				2.2		5.3		3.1					12

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

71.6

2.2

5.3

3.1

NOTES

The 1:1 dilution of the permenant hair dye (without H2O2) was applied at a dose of 18.2mg/cm2. Female, fuzzy Sprague-Dawley Rats were used. For further Experimental Detail see J. Soc. Cosmet. Chem. 48 187-197 (1997) The % absorption was given as +/- 0.52SE, but I cannot convert this to SD for the database as they don't give an exact number of experiments but simply say for 4-5 replicates for 3 rats. I have entered the lowest possible number of replicates as the value for n.

REFERENCE

Percutaneous Penetration And Metabolism Of 2-Nitro-p-phenylenediamine In Human And Fuzzy Rat Skin (2000) Toxicol. Appl. Pharmacol., 166 :13-23