IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 697
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Dibutylphthalate
1050
Infinite
50
None (Neat)
Rat
Full Thickness Skin
Dorsal
1.76
Static
RPMI & 2% bovine albumin & 1% antibiotics
36
Water Bath
24
24
1
Radiloabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
39
1
18
NOTES
Occluded by the infinite dose. Sprague-Dawley Rats were used.
REFERENCE
In Vivo And In Vitro Percutaneous Absorption Of [14C]Di-N-Butylphthalate In Rat (2001) Drug Met. Dispos., 29 :843-853
