IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 70
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Testosterone
0.006
Petrolatum
Testskin TM
3microm pore size polycarbonate membrane
N/A
0.8
Static
LDE Assay Medium & gentamycin sulphate
32
Water Bath
6
6
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.0016
12
NOTES
0.5 microCi and 2microg of testosterone in 0.15g petrolatum. The Kp was calculated from the average % applied dose/cm2/h for the 2nd through to the 6th h, when rates had reached steady state. 3 LSE's per exp 3-6 exp.
REFERENCE
Absorption And Metabolism Of Topically Applied Testosterone In An Organotypic Skin Culture (1992) Skin Pharmacol., 5 :146-153