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In Vitro Study No: 709

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Diazinon0.190.010.0095AcetoneTestskin TMFull Thickness SkinCultured Human Foreskin0.2Flow-throughSterile Ringers Solution, Trizma, glucose & antibioltics37Incubator48482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
87.61.13 0.314.3 13.3 91.226014 41.6

NOTES

Testskin came from Organogenesis Inc. Cambridge, MA, USA. The % Penetrated was calculated from adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption. The acetone vehicle was evaporated from the dose sites using a cool air current. Extra Experimental Detail was gained from Refs 88 + 90

REFERENCE

In Vitro Dermal Absorption Of Pesticides IV. In Vivo And In Vitro Comparison with the Organophosphorus Insecticide Diazinon In Rat, Guinea Pig, Pig, Human And Tissue-Cultured Skin (1994) Toxicol. Vitro, 8 :1213-1218