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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 71

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Beta-Estradiol

Water

Testskin TM

3microm pore size polycarbonate membrane

N/A

0.5

Static

LDE Assay Medium & gentamycin sulphate

Water Bath

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.019	12

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.019

NOTES

2microCi and 2microg of estradiol in 1ml water. The Kp was calculated from the average % applied dose/cm2/h for the 2nd through to the 6th h, when rates had reached steady state. 3 LSE's per exp 3-6 exp.

REFERENCE

Absorption And Metabolism Of Topically Applied Testosterone In An Organotypic Skin Culture (1992) Skin Pharmacol., 5 :146-153