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In Vitro Study No: 712

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
N,N-Diethyl-m-toluamide0.250.010.0125AcetoneGuinea pigDermatomed to 0.5mmBack0.2Flow-throughSterile Ringers Solution, Trizma, glucose & antibioltics37Incubator24482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
89.22.63 3.3315.2 26.1 10.91.40.40.14 41.3

NOTES

Hairless Female Guinea-Pigs were used. The % Penetrated was calculated from adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption. The acetone vehicle was evaporated from the dose sites using a cool air current. Extra Experimental Detail was gained from Ref 90

REFERENCE

An Automated In Vitro Dermal Absorption Procedure: III. In Vivo and In Vitro Comparison With The Insect Repellent N,N-Diethyl-m-Tolumamide In Mouse, Rat, Guinea Pig, Pig, Human And Tissue Cultured Skin (1993) Toxicol. Vitro, 7 :167-176