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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 72

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Hydrocortisone

Water

Testskin TM

3microm pore size polycarbonate membrane

N/A

0.5

Static

LDE Assay Medium & gentamycin sulphate

Water Bath

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00056	12

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00056

NOTES

2microCi and 2microg of hydrocortisone in 1ml water. The Kp was calculated from the average % applied dose/cm2/h for the 2nd through to the 6th h, when rates had reached steady state. 3 LSE's per exp 3-6 exp.

REFERENCE

Absorption And Metabolism Of Topically Applied Testosterone In An Organotypic Skin Culture (1992) Skin Pharmacol., 5 :146-153