EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 728

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
DDT0.3240.010.0162AcetoneTestskin TMFull Thickness SkinCultured Human Foreskin0.2Flow-throughSterile Ringers Solution, Trizma, glucose & antibioltics37Incubator24482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
93.34.83 7.2112.8 13 0.20.07   4 

NOTES

Testskin came from Organogenesis Inc. Cambridge, MA, USA. Extra Experimental Detail was obtained from reference number 88. The % Penetrated was calculated from adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption. The acetone vehicle was evaporated from the dose sites using a cool air current.

REFERENCE

In Vitro Dermal Absorption Of Pesticides VI. In Vivo And In Vitro Comparison Of The Organochlorine Insecticide DDT In Rat, Guinea Pig, Pig, Human and Tissue Cultured Skin (1994) Toxicol. Vitro, 8 :1225-1232