EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 732

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
DDT1.42720.010.0223AcetoneTestskin TMFull Thickness SkinCultured Human Foreskin0.64Flow-throughHanks HEPES Buffered medium, 4% BSA & gentamycin37Incubator24482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
100.12.99 0.5510.3 10.6 0.30.02101 4 

NOTES

Testskin came from Organogenesis Inc. Cambridge, MA, USA. For More Experimental Detail See Bronaugh RL, (1991) A flow-through diffusion cell. In In Vitro Percutaneous Absorption: Principles, Fundamentals, and Applications. Edited by R.L Bronaugh and HI Maibach. Pp. 223-230 CRC Press.% Penetrated was calculated from adding together the amount found in the receptor fluid to the amount found in the skin digest at the end of the experiment. The skin was washed with an aqueous radiac soap solution at 24 hours and immediately after that there was an increase in absorption

REFERENCE

In Vitro Dermal Absorption Of Pesticides VI. In Vivo And In Vitro Comparison Of The Organochlorine Insecticide DDT In Rat, Guinea Pig, Pig, Human and Tissue Cultured Skin (1994) Toxicol. Vitro, 8 :1225-1232