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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 736

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2-Butoxyethanol90052500None (Neat)HumanIsolated EpidermisAbdomen1.8StaticDistilled Water30Not Given881GC with flame ionisation detection

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          1987000.000214  

NOTES

An integrity test was conducted with tritiated water. It was as if the cell were occluded due to the infinite dose. The lag time was less than 1 hour. I have not included the number of experiments performed as it is unclear from the paper. The experiment that I have entered into the database is using vertical static cells. But they also ran some using horizontal cells with 1ml of donor solution.

REFERENCE

Absorption Of Some Glycol Ethers Through Human Skin In Vitro (1984) Environ. Health. Perspect., 57 :193-197